Candida albicans Adhesion on Denture Base Resin Treated with Liquid-Polish

Objective: This study aimed to quantify Candida albicans biofilm formation and to evaluate the surface integrity of a denture base resin treated with liquid-polish Biscover LV (Bisco, Schaumburg, USA) after 30 and 90 cycles of chemical disinfection. Method: Specimens were prepared, sterilized and randomly divided into 15 groups (n=14), according to the type of polishing (mechanical 0.3 or 3 µm of roughness and Biscover LV), number of cycles tested (0, 30, 90) and the solution used (none, 1% sodium hypochlorite and distilled water). In each group, twelve samples were analyzed by the method of crystal violet staining for biofilm quantification. The specimens were exposed to the assigned solutions, immersed in artificial saliva (2h) and inoculated with 2mL of C. albicans (1.10⁷ cel/mL, 90min). Afterwards, samples were washed and immersed in TSB (24h, 37°C) to biofilm growth. This analysis was performed by a spectrophotometer at a wavelength of 595nm by measuring the level of absorbance obtained after the biofilm’s stain release. For qualitative analysis of the liquid-polish layer integrity, two samples from each group (one colonized and one not) were photographed in six different fields by scanning electron microscope (SEM). Data were analyzed (ANOVA, Tukey’s, p<0.05). Result: There was no significant difference between 0.3µm mechanical polished group and liquid-polished group when compared within the same time and tested solutions. However, when comparing these two groups to the 3µm mechanical polished group there was statistical difference. SEM analysis of the colonized specimens demonstrated the presence of C. albicans in the yeast and hyphae form in all groups. Specimens that were not colonized showed a progressive degradation that seemed greater when number of cycles and chemical challenges were increased. Conclusion: Liquid-polish groups showed an amount of biofilm similar to a smooth surface (0.3µm) justifying its clinical use in denture’s rough surfaces.