IADR Abstract Archives

Apoptosis Evaluation in the Inflammatory Infiltrates Of Oral Lichen Planus

Objective: Studies on apoptosis in Oral Lichen Planus (OLP) have been focused on the epithelium. The role of apoptosis regulating lymphocyte cell death within inflammatory infiltrates of Oral Lichen Planus (OLP) has not been elucidated yet. The aim of this study was to evaluate apoptosis in the inflammatory infiltrates of OLP using immunohistochemistry. We also investigated possible associations between apoptosis and the number of CD8+ T lymphocytes and the clinical manifestations of the disease.

Method: Samples of reticular OLP (15), erosive OLP (15) and inflammatory fibrous hyperplasia (15-control) were employed. Immunohistochemical analyses were performed to evaluate the expression of caspase-8, caspase-3, Bax, and Bcl2, as well as the presence of CD8+ T lymphocytes.

Result: No immunostaining was detected for caspases-8 and 3 in the inflammatory infiltrates of erosive and reticular OLP. In contrast, both caspases had an intense immunoexpression in the control group. Bax was not detected in any of the groups studied. The index of CD8+ and Bcl2+ cells was higher in the OLP samples than in the control group. Furthermore, the number of Bcl2+ cells was significantly higher in the erosive than in the reticular type of OLP.

Conclusion: The data suggest that the intrinsic apoptotic pathway is being blocked by Bcl2 expression in the inflammatory infiltrates of OLP, especially in the erosive type contributing for the persistence of inflammation and evasion of apoptosis in that lesion.

Division: IADR/AADR/CADR General Session
Meeting: 2013 IADR/AADR/CADR General Session (Seattle, Washington)
Location: Seattle, Washington
Year: 2013
Final Presentation ID: 2239
Abstract Category|Abstract Category(s): Oral Medicine & Pathology
Authors
  • Ferreira De Aguiar, Maria Cássia  ( Universidade Federal de Minas Gerais, Belo Horizonte, N/A, Brazil )
  • Brant, Juliana  ( Universidade Federal de Minas Gerais, Belo Horizonte, N/A, Brazil )
  • Cotta Ribeiro, Daniela  ( Universidade Federal de Minas Gerais, Belo Horizonte, N/A, Brazil )
  • Campos, Paula  ( Universidade Federal de Minas Gerais, Belo Horizonte, N/A, Brazil )
  • César Vasconcelos, Anilton  ( Universidade Federal de Minas Gerais, Belo Horizonte, N/A, Brazil )
  • SESSION INFORMATION
    Poster Session
    Oral Mucositis and Therapy
    03/22/2013