Method: Nine examinees were checked up their dental condition prior to this study. Also two different types of toothbrushes were distributed to each member. Toothbrush (A) has a standard head and (B) has a small head. Before starting this examination, four pieces of plastic panels (210×297mm) were prepared. Then each panel was sat a 40cm distance in front and to the sides of examinee’s face. One was central and two of them were symmetrically 45 degrees to the side of the face. The final panel was sat below the face horizontally. As the baseline examination, each panel was measured for contamination on the surface by AST, before brushing. Following this examination, each examinee brushed their teeth without toothpaste for 3 minutes in front of their panel-set. After brushing, AST was taken and this data was compared with their baseline data.
Result: The result of AST showed a clear change between before and after brushing. The average of the baseline data (A: 842± 879RLU, B: 1700±2232) showed a notable increase after brushing (A: 21119±26616, B: 11022±15101). There was a statistically significant differences (p<0.05). Additionally, the increase of AST data showed that A (20277RLU) was a statistically higher than B (9322 RLU) (p<0.05).
Conclusion: The results of this study showed significant relationships between brushing teeth and environmental pollution. This evidence demonstrated that AST would be a useful indicator of environmental preservation for treatment space.