To investigate the diffusion through dentine and antimicrobial effect of new intracanal preparations against E. faecalis
Method:
2% clorhexidine digluconate gel, 3% nitrofurantoin gel and 3% doxycycline hydrochloride gel were prepared and placed inside root canal. After seal of apical and coronal openings, the teeth were placed on Muller Hinton agar, where E. faecalis was previously seeded. After 24 h of incubation period at 36C, the zone of inhibition of microbial growth was measured.
Result:
The antimicrobial properties of these formulations can be ranked from the high to the low effect: 3% doxycycline hydrochloride gel, 2% clorhexidine digluconate gel and 3% nitrofurantoin gel. Significant differences between groups were observed by the Kruskal-Wallis test.
Conclusion: The intracanal formulations with doxycycline and chlorhexidine diffused better into the dentin and reached the outer surface, showing antimicrobial effect against E. faecalis.