Proteome and Peptidome of Acquired Enamel Pellicle on Deciduous Teeth
Understanding the composition and structure of the acquired enamel pellicle (AEP) has been a major goal in oral biology. Our lab has conducted studies on the composition of AEP formed on permanent enamel. The exhaustive exploration has provided a comprehensive identification of more than 100 proteins from AEP formed on permanent enamel. The AEP formed on deciduous enamel has not been subjected to the same biochemical characterization scrutiny as that of permanent enamel, despite the fact that deciduous enamel is structurally different from permanent enamel. Objective: We hypothesized that AEP proteome and peptidome formed on deciduous enamel may also be composed of unique proteins, some of which may not be common with AEP of permanent enamel explored previously. Method: In vivo pellicle material was collected from 10 children (aged 18-54 months) and subjected to classical biochemical methods and state-of-the art mass spectrometry analysis. Result: A total of 76 pellicle proteins were identified from the deciduous pellicle proteome. In addition, 38 natural occurring AEP peptides were identified from 10 proteins, suggesting that primary AEP proteome/peptidome presents a unique composition.Conclusion: This is the first study to provide a comprehensive investigation of in vivo AEP formed on deciduous enamel.The primary AEP proteome/peptidome demonstrated different trends when compared with the permanent AEP proteome/peptidome. This study emphasizes the importance of investigating the physiological conditions of the AEP on different enamel substrates (permanent and primary teeth). In consequence of different AEP composition between primary and permanent teeth, clinically the mechanism behind the initiation of oral pathologies related to this protein film could be different. In addition, our study provides a foundation for future studies that can translate into better therapeutics aimed towards children. CIHR grant# 106657, grant# 97577 and grant# 113166 and CFI-LOF grant# 25116.JNZ is recipient of a ODA an CIHR Schulich Dentistry Scholarship. YHL is recipient of a CIHR undergraduate scholarship.
Division: IADR/AADR/CADR General Session
Meeting:2013 IADR/AADR/CADR General Session (Seattle, Washington) Location: Seattle, Washington
Year: 2013 Final Presentation ID:2607 Abstract Category|Abstract Category(s):Salivary Research
Authors
Zimmerman, Jason Noah
( University of Western Ontario, London, ON, Canada
)
Custodio, William
( Western University, London, ON, Canada
)
Hatibovic-kofman, Sahza
( University of Western Ontario, London, ON, Canada
)
Lee, Young Ho
( University of Western Ontario, London, ON, Canada
)
Xiao, Yixhi
( University of Western Ontario, London, ON, Canada
)
Siqueira, Walter L.
( Western University, London, ON, Canada
)