Method: Bovine enamel blocks (n=90) were subjected to short-term acidic exposure by immersion in citric acid 0.05 M (pH 2.5) for 15 s, resulting in surface softening without tissue loss. Than 75 selected eroded enamel blocks were randomly and equally divided into 5 groups according to saliva formulation (n=15): C- control (deionized water); S1- Klimek et al., 1982 (0.33g KH2PO4, 0.34g Na2HPO4, 1.27g KCl, 0.16g NaSCN, 0.58g NaCl, 0.17g CaCl2, 0.16g NH4Cl, 0.2g urea, 0.03g glucose, 0.002g ascorbic acid, 2,7g mucin in 1,000 mL distilled water/pH7); S2- Bartlett et al (0,1029g CaCl2 2H2O, 0, 04066g MgCl2, 0,544g KH2PO4, 4,766g Hepes buffer acid form, 2,2365 KCl); S3- Lussi et al (0.381g NaCl, 0.213g CaCl2·2H2O, 1.114g KCl, 0.738g KH2PO4, and 2.2g mucin in 1,000 mL distilled water/pH7); S4- Amaechi et al (2g methyl-p-hydroxybenzoate, 10g sodium carboxymethyl cellulose, 0.625g KCl, 0.059g MgCl2.6H2O, 0.166g CaCl2.2H2O, 0.804g K2HPO4 and 0.326 g KH2PO4 in 1000 ml of water); After demineralization enamel blocks were subjected to remineralization by immersion in the saliva’s formulation for 2 h. Enamel remineralization was measured by superficial microhardness test (% superficial microhardness change). The data were tested using Kruskal-Wallis and Tukey’s test (p<0.05).
Result: All the tested formulations of artificial saliva resulted in significantly higher enamel remineralization, without differences among them, when compared to control group of deionized water.
Conclusion: Besides the variety of artificial saliva for erosion in vitro protocols, the formulations tested in the present studied showed similar potential to remineralize softened erosion enamel lesions.