To detect micro-particles of EndoSquence and MTA in liver and kidney after subcutaneous implantation and correlate this with the systemic adverse effect using Environmental Scanning Electron Microscope (ESEM) and Energy Dispersive X-ray analysis (EDX).
Method:
Thirty-six albino rats were divided randomly into three equal groups (n=12). Group (1); the control group, received empty sterile Teflon tubes. Group (2); the MTA group, received Grey ProRoot MTA. Group (3); the EndoSequence group, received EndoSequence root repair material. The materials in the Teflon tubes were implanted subcutaneously in the dorsal side. Half of the rats were sacrificed after 7 days and the other half were sacrificed after 30 days. The organs were then prepared for histopathological examination and number of inflammatory cells was calculated ; 20µm sections of liver and kidney and the materials inside the retrieved Teflon tubes were exposed to EDX analysis.
Result:
Micro-particles size 1.5-5µm were detected in all samples of MTA and EndoSequence .EDX analysis showed Ca, Si, Al, Mg, Na, and Fe peaks for the MTA group while in the EndoSequence group, Ca, Si, Al, Mg and Fe peaks were displayed and matched with the analysis of the materials inside the tubes. No inorganic micro particles were detected in the control group. Histopathological results showed that both materials had a significantly more inflammatory reaction than the control group after 7days(p˂0.05) and decreased after 30 days.The MTA group had a significantly more inflammatory reaction than the EndoSequence (p˂0.05).
Conclusion:
The Micro-particles of MTA and EndoSequence left the surgical site and travelled via the blood stream to reach the liver and kidney and caused an adverse effect. The MTA micro particles seemed to be more toxic for rat’s liver and kidney than EndoSequence .