Objective: The aim of this study was to evaluate the bone remodeling process in palatal suture during SD in rats with streptozotocin (STZ)-induced type 1 DM.
Method: Ninety Wistar male rats were randomly assigned to six groups with 15 animals each: DM-STZ induced (D), Normal (N), citrate buffer (C), DM-STZ induced with SD (D+SD), normal with SD (N+SD), citrate buffer with SD (C+SD). The animals were euthanized at 3, 7 and 10 days after disjunction. Real-Time RT-PCR was used to quantify the expression of mRNAs for osteoprotegerin (OPG), RANK, RANKL, osteonectin (ONC), osteocalcin (OCC), bone sialoprotein (BSP), osteopontin (OPN) and bone morphognetic protein-2 (BMP-2) genes along with the changes in body weight and water intake. Data were submitted to statistical analysis using one-way ANOVA followed by Tukey test (alpha= 0.05).
Result: Quantitative RT-PCR revealed that gene expression involved in bone healing was differentially modulated in D and D+SD groups compared to N, C, N+SD and C+SD groups (p < 0.05), regarding mRNAs for OPG, RANK, RANKL, ONC, OCC, BSP, OPN and BMP-2.
Conclusion: Our results show that DM modulates differential gene expression on bone palatal suture, indicating that these disease might jeopardize the overall reparative response of the palatal suture tissue during bone remodeling after SD. Financial supported by CAPES and FAPESP.