Method: Subgingival samples were collected with sterile paper in 151 patients exhibiting different periodontal conditions. The occurrence of P. gingivalis, FimA genotypes and other bacteria was determined by PCR. The Chi-squared test was used, we applied the Wilcoxon and Mann - Whitney test and T test for quantitative variables.
Results: Porphyromonas gingivalis was positive in 85 patients, (52% for healthy patients, 59.7% for gingivitis patients and 53.1% for chronic periodontitis patients). In both healthy and sick subjects was more prevalent genotype FimA II, and there was no statistically significant differences between the 3 groups. FimA IV was more prevalent in gingivitis patients (13%), and FimA V was not detected in the studied population. There was a high correlation (P = 0.000) between P. gingivalis, T. denticola and T. forsythia. Genotype FimA II was found in a high percentage of correlation with this bacteria.
Conclusion: The prevalence of P. gingivalis in the present study is high in 3 groups. The distribution of genotypes in different periodontal diagnosis was heterogeneous, and there was no statistically significant differences. However, a trend toward greater frequency of FimA II genotype in patients with moderate gingivitis and severe periodontitis and this genotype was associated with increased pocket depth and greater loss of attachment level. There was a relationship of co-infection by P. gingivalis, T. denticola and T. forsythia, FimA II genotype being the most frequent in the samples which were presented this co-infection.