Method: Subconfluent SGT cell cultures have been treated with CsA at in vivo relevant concentrations for 24h. MTT assay for cellular proliferation of cultured SGT cell line has been performed and TGase 1 activity assay for cellular differentiation has been detected in the CsA-treated samples.
Result: MTT assay of SGT cell line showed about 95% cell viability at 0.1 μg/ml CsA, about 65% at 5.0 μg/ml and 35% at 10 μg/ml CyA (Fig. 1). Growth curve showed slower growth pattern under higher CsA concentration than that of control group. Growth rate under 10μg/ml CsA prominently decreased after 1 day. TGase 1 activity showed about 3×103 cpm/h/mg protein under pre- and postconfluency at control and lower concentration, while was decreased at higher concentration. TGase 1 activity under preconfluency showed no prominent change according to 1.0, 5.0 and 10 μg/ml of CyA. And also there was no change under postconfluency according to 1.0, 5.0 and 10 μg/ml of CyA .
Conclusion: It suggested that CsA could have an inhibitory effect in the proliferation of SGT cell line but no in the differentiation.