Abstract Archives

IADR Abstract Archives

Cytotoxicity of monomers and plant extracts on pulp fibroblasts

Objective: Endodontic sealers remain for long periods in contact with the tissues, and depending on their chemical constitution, these periods may be indeterminate. Therefore, it is necessary that materials be well tolerated by periapical tissues and, if possible, assists in the repair process, and allows good and longer sealing the root canal system. The aim of this study was evaluate the cytotoxicity by XTT and rhodamine B of monomers and natural extracts present in sealers on primary human pulp fibroblasts. 

Method: BisGMA, UDMA, Copaiba oil resin and castor oil polymer were dissolved in DMSO and sequentially diluted to obtain different concentrations of stock solution prior to its addition into culture medium. Pulp human fibroblasts were seeded into 96-well culture following 24 h of incubation and then the cells were exposed with conditioned medium. After 24h the cell viability were evaluated by XTT and SRB tests. The difference among cell survival rates were statistically analyzed by ANOVA e Tukey (p <0.05).

Result: All material showed significant difference if compared to untreated control. Cytotoxicity ranking for XTT and SRB were the same: BisGMA > Copaíba ≥ UDMA> Mamona.

Conclusion: Of all materials, castor oil polymer had the lower cytotoxicity. These results are useful to understand and develop new endodontic materials with low toxicity.

Division: IADR/AADR/CADR General Session
Meeting: 2013 IADR/AADR/CADR General Session (Seattle, Washington)
Location: Seattle, Washington
Year: 2013
Final Presentation ID: 1110
Abstract Category|Abstract Category(s): Dental Materials 5: Biocompatibility and Biologic Effects
Authors
  • Pavanello, Marcella  ( Universidade Est. Paulista Julio Mesquita, Sao Jose dos Campos, N/A, Brazil )
  • Silva, Gleyce Oliveira  ( Universidade Est. Paulista Julio Mesquita, Sao Jose dos Campos, N/A, Brazil )
  • Valera, Marcia Carneiro  ( Universidade Est. Paulista Julio Mesquita, Sao Jose dos Campos, N/A, Brazil )
  • Cavalcanti, Bruno  ( Universidade Est. Paulista Julio Mesquita, Sao Jose dos Campos, N/A, Brazil )
  • Camargo, Carlos H. R.  ( Universidade Est. Paulista Julio Mesquita, Sao Jose dos Campos, N/A, Brazil )
    • SESSION INFORMATION
    Poster Session
    Toxicity
    03/21/2013