Co-Culture of EPC and osteoblasts on modified Titanium surfaces
Objectives: Obligate precondition for successful dental implant osseointegration is the development of timely and sufficient periimplant neovascularisation and osteoblasts differentiation. It is widely accepted that especially endothelial progenitor cells (EPC) play an essential role to trigger blood supply during soft and hard tissue regeneration. Objective of our proposal is to test the hypothesis that osteoblasts on titanium surface can positive influenced by endothelial progenitor cells (EPC).
Methods: Osteoblasts and EPC were plated on titanium surface. On day one, day three and day seven, cell number of both cell types, expression profile e.g. Type-I-collagen, alkaline phosphatase, etc. were measured by RT-PCR. Cell morphology was assessed by confocal-laser-scanning-microscopy (CLSM).
Results: In contrast to osteoblast-mono-culture, osteoblast-EPC-co-culture increased significantly the differentiation of osteoblasts.
Conclusion: We demonstrate the first time a co-culture analysis of osteoblast-EPC-co-culture on titanium surface. The results underline the importance of angiogenesis and neovascularization by EPC for osteoblast differentiation and therefore osseointegration.
Division: IADR/AADR/CADR General Session
Meeting:2013 IADR/AADR/CADR General Session (Seattle, Washington) Location: Seattle, Washington
Year: 2013 Final Presentation ID:883 Abstract Category|Abstract Category(s):Oral & Maxillofacial Surgery
Authors
Ziebart, Thomas
( Universität Mainz, Mainz, , Germany
)
Boddin, Alexander
( Universitätsmedizin Mainz, MAinz, N/A, Germany
)
Pabst, Andreas Max
( University Mainz, Bruchsal, N/A, Germany
)
Ziebart, Johanna
( Universitatsklinik f. Zahn-, Munund Kieferheilkunde, Mainz, N/A, Germany
)
Jacobs, Collin
( University Mainz, Mainz, N/A, Germany
)
Walter, Christian
( Universitätsmedizin Mainz, Mainz, N/A, Germany
)
SESSION INFORMATION
Poster Session
Oral & Maxillofacial Surgery I
03/21/2013