Method: Synthesis and characterization of silver nanoprisms and nanospheres by: UV-Vis, Raman spectroscopy and TEM. PMMA was synthesized using TiO2 anatase nanoparticles. The TiO2 was characterized by: UV-Vis, FT-IR, Raman-Dispersive, X-Ray Diffraction and TEM. The synthesized PMMA/TiO2 was analyzed by: Raman-Dispersive, TGA and DSC. Discs (n=9) of PMMA/TiO2 were added to silver nanospheres and nanoprisms and thermopolymerized by microwave. Candida albicans adherence and cytotoxic (MTT) assays were assessed. NatureCryl® was used as comparative group. One-Way ANOVA (P<0.05) was applied.
Results: There were proved spectroscopically the presence of silver nanoprisms (95 nm) and nanospheres (10-12 nm). The TiO2 is amorphous, irregular particles (50-130 nm). The PMMA/TiO2 is a polymer with spherical shape (20-30 µm). The Candida albicans adherence was significantly reduced (P<0.05) using PMMA/TiO2with silver nanospheres compared with the achieved effect using silver nanoprisms. All groups showed no cytotoxic and genotoxic effect (P>0.05) when were in contact with mouse fibroblast culture cells.
Conclusion: There was developed an acrylic resin for dentures with lower Candida albicans adherence and with non-cytotoxic effect.
Acknowledgements: Dra. Marina Vega, Q. Carmen Vázquez, Miguel A. Arellano.