IADR Abstract Archives

Effects of Gluma on Dentin MMP Activity

Objective: Dentin matrix is known to contain matrix metalloproteinases (MMPs)-2, 3, 8, 9 and 20.  In mineralized dentin, these MMPs are covered with mineral crystallites and are inactive. However, after acid-etched, they are uncovered and activated and may degrade the matrix. The aim of this study was to investigate the MMP inhibitory effect of glutaraldehyde-containing agents.

Method: Mineralized dentin beams 1 x 1 x 6 mm were prepared from mid-coronal dentin of extracted human third molars.  The beams were dipped in 37 wt% phosphoric acid (15 sec), dipped in water 15 sec, dipped in a treatment solution for 1 min, dipped in water 10 sec and then placed in 400 µL generic MMP substrate (SensoLyte, AnaSpec Inc., Fremont, CA) for 60 min. After 1 hr, the absorbance at 412 nm was read in a 96-well plate reader against blanks and data were statistically analyzed (p<0.05). 

Result:

O.D. 412 nm

Etched

5% GA

5% GA/35% HEMA

Gluma liquid

Gluma gel

0.33 ± 0.05a

0.03 ± 0.02c

0.03 ± 0.01c

0.05 ± 0.03bc

0.06 ± 0.02b

Values are mean±SD (n=10); GA=5 wt% glutaraldehyde; HEMA=2-hydroxyethylmethacrylate; Gluma=Gluma Desensitizer; Heraeus=5% GA + 35% HEMA + 60% water. Different superscript letters indicate significant differences (p<0.05) between groups.

Conclusion: All treatments significantly reduced the total MMP activity of acid-etched dentin after a 1 min treatment.  Thus, Gluma can be used to inactivate MMPs just prior to bonding.  This should increase the durability of resin-dentin bonds.  Supported, in part, by R01DE015306 to DP (PI) and FAPESP 2012/08866-4.

IADR/AADR/CADR General Session
2013 IADR/AADR/CADR General Session (Seattle, Washington)
Seattle, Washington
2013
408
Dental Materials 1: Adhesion - Bond Strength Testing and Mechanisms
  • Hebling, Josimeri  ( Araraquara Dental School - UNESP, Araraquara - SP, , Brazil )
  • Scheffel, Débora L.  ( UNESP - Univ. Estadual Paulista, Araraquara, Sao Paulo, N/A, Brazil )
  • Kious, Andrew  ( Georgia Health Sciences University, Augusta, GA, USA )
  • Furness, Alan  ( Georgia Health Sciences University, Augusta, GA, USA )
  • Tay, Franklin R.  ( Georgia Health Sciences University, Augusta, GA, USA )
  • Pashley, David  ( Georgia Health Sciences University, Augusta, GA, USA )
    • Oral Session
    Dentin Bonding: Effects of Inhibitors and Protease Activity
    03/21/2013