Method: To improve its consistency MTA was added to: AH Plus (Dentsply, Brazil), a Castor oil bean polymer (Polifil - Polymer Chemicals Ltda. Araraquara - SP, Brazil) and MTA Fillapex (Angelus – Londrina, Brazil). The experimental materials were tested for cytotoxicity and solubility using setting times of 0 hours, 24 hours, 7 days and 30 days. Chinese hamster lung fibroblasts (V79) were exposed to different dilutions of the extracts for 24 h and cell viability was measured by MTT assay. For the solubility test, 12 samples of each material were prepared using teflon rings. The samples were incubated at 37° C with 100% relative humidity for 7 days, weighted on precision scale in distilled water, and kept in the incubator at 37 °C for 24h.
Result: The MTA pure and its association with the Castor oil bean polymer were not cytotoxic, with results similar to the control group. The Fillapex + MTA appeared toxic at 1:2 and 1:4 dilutions, only in 0h and 24h; AH Plus was most cytotoxicity in almost all dilutions, with over 50% viability only at 1:16 dilution in all periods. For the solubility test, it was observed that the MTA pure was the only material that differed from the others, with no solubilization. In association with AH Plus, Castor oil bean and Fillapex, had weight loss of 6.1%, 3.0% and 3.6%, respectively.
Conclusion: MTA pure and added to Castor oil bean and Fillapex were similar for cytotoxicity and only MTA had no weight loss.