Method:
30 children aged 3 to 7 years with or without caries were included into study. The super gingival plaques in three different sites, which show three different states of caries: intact enamel, white spot lesions and carious dentin lesions were collected. Supragingival plaque microbiota were obtained by high-throughput barcoded pyrosequencing, and analyzed by SPSS soft ware 19.0.
Result:
A total of 572,773 passed the quality control and 25,444 unique sequences were identified. There are 145 genera belonging to eighteen phyla were found in the plaque samples. The overall framework of healthy oral microbiomes was much more diverse than that in dental caries. Moreover the initiation stages of caries, especially the white spot lesions (Twenty three genera) were more diversity than established caries (cavitated dentin) (twenty one.) It showed that Actinomyces and Stenotrophomonas were significantly associated with white-spot lesions, while other known acid producers such as Streptococcus, Scardovia, Olsenella and Lactobacillus were found at their highest levels in carious dentin lesions.
Conclusion:
Bacterial profiles changed in different caries states. And bacteria shift in the different caries state. The shifts in community structure of plaque microbiota may contribute to cavity formation.