Aberrant Expression of Annexin A10 in Human Oral Cancer
Objectives: Annexins are calcium and phospholipid binding proteins that form an evolutionary conserved multigene family. Considerable evidence indicates that annexin A10 (ANXA10) is involved in tumoral progression, although little is known about its role in human oral carcinogenesis. To determine the possible association between aberrant expression of ANXA10 and the development of oral squamous cell carcinoma (OSCC), we have demonstrated a comprehensive analysis for not only OSCC-derived cell lines but a large scale of clinical samples.
Methods: ANXA10 mRNA and protein expressions were assessed by quantitative reverse transcriptase polymerase chain reaction and immunoblotting. We then conducted a proliferation assay and cell-cycle analysis in ANXA10 knockdown cells in vitro. In addition, we evaluated the correlation between the ANXA10 expression status in 110 primary OSCCs and the clinicopathological features by immunohistochemistry.
Results: ANXA10 mRNA and protein expression levels were up-regulated in all cell lines examined (n = 10, p < 0.05). ANXA10 knockdown cells showed that cellular proliferation decreased by inactivation of extracellular regulated kinase (ERK) (p < 0.05), and cell-cycle arrest at the G1 phase resulted from up-regulation of cyclin-dependent kinase inhibitors. ANXA10 protein expression in primary OSCCs was also significantly greater than in normal counterparts (p < 0.05), and higher expression was correlated with tumoral size (p = 0.027).
Conclusions: These results suggested that ANXA10 plays an important role in cellular proliferation and ANXA10 expression is likely to be a biomarker of progression and a potential therapeutic target for the development of new treatments for OSCCs.
Division: IADR/AADR/CADR General Session
Meeting:2013 IADR/AADR/CADR General Session (Seattle, Washington) Location: Seattle, Washington
Year: 2013 Final Presentation ID:892 Abstract Category|Abstract Category(s):Oral & Maxillofacial Surgery
Authors
Shimizu, Toshihiro
( Chiba University, Chiba, N/A, Japan
)
Shiiba, Masashi
( Chiba University, Chiba, N/A, Japan
)
Tanzawa, Hideki
( Chiba University, Chiba, N/A, Japan
)
Uzawa, Katsuhiro
( Chiba University, Chiba, N/A, Japan
)
Kasamatsu, Atsushi
( Chiba University, Chiba, N/A, Japan
)
Shimizu, Fumie
( Chiba University, Chiba, N/A, Japan
)
Yamamoto, Ayumi
( Chiba University, Chiba, N/A, Japan
)
Koike, Kazuyuki
( Chiba University, Chiba, N/A, Japan
)
Ishige, Shunsaku
( Chiba University, Chiba, N/A, Japan
)
Sakamoto, Yosuke
( Chiba University Hospital, Chiba, N/A, Japan
)
Ogawara, Katsunori
( Chiba University Hospital, Chiba, N/A, Japan
)
SESSION INFORMATION
Poster Session
Oral & Maxillofacial Surgery I
03/21/2013