Methods: A bacterial in vitro experiment, using Streptococcus mutans ATCC and a clinical strain to test 12% chlorhexidine gluconate and silver 30% diamine fluoride (control) and chitosan, chitosan +silver nanoparticles, chitosan +fluoride and chitosan + silver nanoparticles and fluoride ( experimental). The sizes of the silver nanoparticles were between 8 and 10 nanometer
Results: The MIC and MBC for the new product for ATCC strains was ±112.616 µg/mL, for clinical strains was 225.232 and 450.464 µg/mL ( p-value 0.498, 0.993 and 0.498 and 0.244 ). Absorbance results for the new product indicated that it is 16 times more powerful for killing s.mutans than the controls. A pilot study indicated that cavities treated with the new product turn pale yellow.
Conclusions:
The new invention showed MIC and MBC values for nano silver fluoride lower than those for silver diamine fluoride and the product does not stain teeth black. A clinical trial will be the next step, however, a small pilot study indicated positive results.