Modified Titanium Prepared Platelet-Rich Fibrin (T-PRF): A Novel Platelet Concentrate

Methods: Blood samples of 10 healthy male volunteers were collected. Blood samples was obtained by syringe and 10ml blood was transferred to Ti tube for each four group. First group was centrifuged for 20min (20min group), second, third and fourth groups were centrifuged for total 20min. with two-minute (2min  group), five-minute (5min  group) and ten-minute(10min  group)-periods clockwise and counterclockwise at 2700 RPM. Half of each T-PRF clot was processed for SEM evaluation. The other half of each clot was processed for fluorescence microscopy analysis and light microscopy analysis.

 Results: It was observed that fibrin clot was formed in each samples. The 20min, 5min and 10min groups showed well-organized structure under low power field by  hematoxylin  and eosin stain. A flabbier fibrin texture and erythrocyte islets in the fibrin were observed in the 2min group. Under higher magnification of same samples, 10 min  group showed better-organized network with continuous integrity compared to the other groups. With the immunofluorescent staining, the fibrin network appeared mature and dense in the 20min, 5min and 10min groups. Fibrin seemed thicker and better organized in 10min group. SEM examination showed more complex and denser fibrin clusters occurred in 10min group than the other groups. 

Conclusions: This pilot study defines Modified T-PRF (10min group)  as an autogenous platelet- and leukocyte-rich fibrin product with superior fibrin network than T-PRF (20min group). More research on clinical parameters such as resorption time, clinical success of this new product are required.