Methods: Three orthodontic materials were tested(SelectDefense/Element34TechnologyInc; TransbondXT/3MUnitek; Fuji Ortho-LC/GCAmerica). Evaluation of antibacterial properties utilized bacterial growth in liquid media(BG) and agar diffusion(AD) tests. In BG, each material included 100 adhesive discs(3x2mm) divided into groups of five in vials together with 0.5ml of brain-heart-infusion broth and 2.5µl of streptococcus mutans(MS)(n=20). Following incubation(37°C,24hours), bacterial growth was measured using a Spectrophotometer(Genesys 20) at 600nm for optical density(OD). In AG, agar plates were inoculated with 100µl of MS in brain heart infusion agar. Fifteen adhesive discs(6x2mm) of each material were prepared and placed in groups of five in 3 agar plates. Following incubation(37°C,48hours), the agar plates were visually inspected for zones of bacterial inhibition. Results were statistically analyzed with One way ANOVA using Tukey’s HSD test for all multiple comparisons.
Results: In BG, selenium containing resin(SeD) promoted more bacterial growth(OD=0.427/StandardDeviation(SD)==0.081) compared to the standard-resin(XT)(OD= 0.222/SD=0.083) and RMGI(OD=0.275/SD=0.093) but less growth compared to the positive-control(OD=0.522/SD=0.070). The differences were statistically significant(p