IADR Abstract Archives

Correlation of 3-D biofilm structure with varying resin composite topographies

Objectives: To determine correlations between the 3-dimensional structures of Streptococcus mutans biofilms with varying resin-composite topographies produced by polishing. Methods: Specimens of Point 4 (Kerr Corp.) resin-composite were sequentially prepared (180-1200grit SiC-disks followed by polycrystalline-diamond suspensions) with final polishes equivalent to 5, 3, 1 or 0.5μm (dimensions d=6mm, h=3mm). Control group specimens were prepared against Mylar^ª strips and left unpolished. Surfaces were imaged using a confocal laser scanning microscope (CLSM) [Leica TCS-NT, reflection mode; three locations/specimen] before UV sterilization. Streptococcus mutans (strain UA159) biofilms were grown microaerophilically on specimens (batch culture, 0.5X Tryptone-yeast-extract medium with 10mM sucrose, 37°C, 100rpm; media replenished after 24h). After 48h bacterial growth, specimens were stained [Concanavalin A-Alexa Fluor 647 conjugate (AF; extracellular polymeric-substances), Sypro Red (SR; proteins), Syto 9 (SY; nucleic-acids); Invitrogen]. Biofilms were imaged using a Leica TCS-SP2-MP CLSM [transmission mode; three comparable locations/specimen]. Topographical parameters (Leica software;v2.61) calculated were: mean surface roughness (R_a), average maximum height (R_p) and average maximum depth (R_v). Biofilm structural parameters (ISA3D software) calculated were: porosity (PR), mean biomass thickness (BT) and bioroughness coefficient (BR). Parameters were analyzed for correlations with polishing groups using Pearson's Correlation, and by separate one-factor ANOVA tests per parameter (α=0.05).

Results:

Group

Topography

Biofilm Structure

R_a (nm)

R_p (μm)

R_v (μm)

PR (μm³)

BT (μm)

5um

588.6±396.4^A

3.3±2.6^A

5.6±5.4^A

0.96±0.01^A,B

44.0±16.0^A

0.83±0.13^A

3um

638.9±323.4^A

3.1±0.8^A

4.2±1.5^A

0.97±0.01^A

32.3±13.0^B

0.73±0.14^B

1um

450.9±391.3^A

2.6±1.5^A,B

4.1±1.8^A

0.96±0.02^B,C

24.1±4.4^C

0.62±0.08^C

0.5um

228.0±119.7^B

1.8±0.6^B

3.3±2.0^A

0.95±0.01^C

34.6±7.3^B

0.61±0.10^C

Control

605.1±331.6^A

2.9±1.5^A

6.3±7.2^A

0.93±0.02^D

26.4±5.9^C

0.68±0.10^B,C

Different superscript letters within columns denote statistically different mean values (p<0.05)

Conclusions: Polishing of the resin-composite produced distinctly different topographies that demonstrated a positive correlation with 48h S. mutans biofilm structures (r ³ 0.45).

Division: IADR/AADR/CADR General Session
Meeting: 2013 IADR/AADR/CADR General Session (Seattle, Washington)
Location: Seattle, Washington
Year: 2013
Final Presentation ID: 609
Abstract Category|Abstract Category(s): Dental Materials 5: Biocompatibility and Biologic Effects

Authors

Khajotia, Sharukh ( University of Oklahoma Heath Science Center, Oklahoma City, OK, USA )

Smart, Kristin ( University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA )

Muckelrath, Halie ( University of Oklahoma Heath Science Center, Oklahoma City, OK, USA )

Hall, Carlie ( University of Oklahoma Heath Science Center, Oklahoma City, OK, USA )

SESSION INFORMATION

Poster Session
Biofilm and Anticaries
03/21/2013