IADR Abstract Archives
Comparison of bone defects and mesenchymal stem cells population in ligature-induced young and old agedperiodontitis mice models
Objectives: The prevalence of periodontitis with bone destruction increases along with aging. It is also well known that one of the critical hallmarks of the aging is exhaustion and disability of mesenchymal stem cells (MSCs) in peripheral tissue. Here, we examined severity of periodontal bone destruction in different age mice and correlation between the number of the MSCs and the bone destruction.
Methods: A periodontitis model was developed in C57BL/6J female mice (5 and 50-week old, n=3 in each age) by ligatingtheir mandibular 1stmolarswith silk threads as described by Abe Tand Hajishengallis G(2013). Mice were sacrificed at 0-, 3- and 10-day after ligation and those mandibles were fixed with 4 % paraformaldehyde. In the micro CT image, relative bone defect area (%) in the furcation area was measured by ImageJ software. To check the distribution of MSCs, the PDGFRapositive cells were counted in the immunohistochemical staining image of the furcation area of 1stmolar.
Results: The relative bone defect area (%) in the periodontitis model was significantly higher in the 50-week old group compared to the 5-week old group (p<0.01, one way ANOVA/ Turkey) at 10-day after ligation. In addition, the bone destruction could be observed even in 3-day after ligation in 50-weeks old group while 5-weeks group showed no bone resorption. In the histological analysis, 50-week old groupshowed lower number of PDGFRapositive cells (112±21.13, 169±17)compared to the 5-week old group (178±16.8, 257±80)of periodontitis models at 3-day and 10-day after ligation respectively.
Conclusions: In this study, old aged mice showed lower mesenchymal stem cell population and more severe bone defects compared to young aged mice.
Methods: A periodontitis model was developed in C57BL/6J female mice (5 and 50-week old, n=3 in each age) by ligatingtheir mandibular 1stmolarswith silk threads as described by Abe Tand Hajishengallis G(2013). Mice were sacrificed at 0-, 3- and 10-day after ligation and those mandibles were fixed with 4 % paraformaldehyde. In the micro CT image, relative bone defect area (%) in the furcation area was measured by ImageJ software. To check the distribution of MSCs, the PDGFRapositive cells were counted in the immunohistochemical staining image of the furcation area of 1stmolar.
Results: The relative bone defect area (%) in the periodontitis model was significantly higher in the 50-week old group compared to the 5-week old group (p<0.01, one way ANOVA/ Turkey) at 10-day after ligation. In addition, the bone destruction could be observed even in 3-day after ligation in 50-weeks old group while 5-weeks group showed no bone resorption. In the histological analysis, 50-week old groupshowed lower number of PDGFRapositive cells (112±21.13, 169±17)compared to the 5-week old group (178±16.8, 257±80)of periodontitis models at 3-day and 10-day after ligation respectively.
Conclusions: In this study, old aged mice showed lower mesenchymal stem cell population and more severe bone defects compared to young aged mice.