IADR Abstract Archives
Surface Deposition Analysis of Bioactive Restorative Material and Cement
Objectives: To investigate the surface deposition of calcium and phosphorous from a bioactive restorative material and luting cement.
Methods: The bioactivity of ACTIVA™ Bioactive-Restorative A2 shade (Pulpdent) and ACTIVA™ Bioactive-Cement (Pulpdent) were studied. Disc-shaped specimens of each material (N=3) were created with the dimensions of 4 mm in thickness and 5 mm diameter by placing the materials in a polypropylene mold and photo-polymerizing for 40 sec on each side. The specimens were removed and polished on 200 Grit SiC paper. Two specimens of each material were hung vertically in 10 mL Dulbecco’s phosphate-buffered saline (PBS) for specified times of 14 or 28 days. The specimens were removed and the buffered saline solution was thoroughly washed off with distilled water to remove adhering foreign ions on the surface. The remaining specimens of each material served as the control samples and were polished and rinsed with saline, but were not treated in PBS. Samples were placed on ½ inch SEM Al stubs with double-side adhesive tape. The stubs were sputter-coated using an Au-Pd target from a Denton Vacuum Desk II sputter coater for 80 seconds. The specimen surfaces were then subjected to Amray 3300 FESEM (SEMTech (STS)) field scanning electron microscope (SEM) imaging and PulseTor SDD (Silicon Drift Detector) Energy-dispersive X-ray spectroscopy (EDS) analysis to determine the structural morphology and the characterization of precipitated material on the surface.
Results: Please see table 1 and 2.
Conclusions: The surfaces of both materials showed higher concentrations of calcium and phosphorous at 14 days and 28 days when compared to their respective controls.
Methods: The bioactivity of ACTIVA™ Bioactive-Restorative A2 shade (Pulpdent) and ACTIVA™ Bioactive-Cement (Pulpdent) were studied. Disc-shaped specimens of each material (N=3) were created with the dimensions of 4 mm in thickness and 5 mm diameter by placing the materials in a polypropylene mold and photo-polymerizing for 40 sec on each side. The specimens were removed and polished on 200 Grit SiC paper. Two specimens of each material were hung vertically in 10 mL Dulbecco’s phosphate-buffered saline (PBS) for specified times of 14 or 28 days. The specimens were removed and the buffered saline solution was thoroughly washed off with distilled water to remove adhering foreign ions on the surface. The remaining specimens of each material served as the control samples and were polished and rinsed with saline, but were not treated in PBS. Samples were placed on ½ inch SEM Al stubs with double-side adhesive tape. The stubs were sputter-coated using an Au-Pd target from a Denton Vacuum Desk II sputter coater for 80 seconds. The specimen surfaces were then subjected to Amray 3300 FESEM (SEMTech (STS)) field scanning electron microscope (SEM) imaging and PulseTor SDD (Silicon Drift Detector) Energy-dispersive X-ray spectroscopy (EDS) analysis to determine the structural morphology and the characterization of precipitated material on the surface.
Results: Please see table 1 and 2.
Conclusions: The surfaces of both materials showed higher concentrations of calcium and phosphorous at 14 days and 28 days when compared to their respective controls.
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