Analysis of cadmium in the saliva by cyclic voltammetry
Toxicology is an area of clinical and laboratory analysis that has gained more relevance since the second half of last century to contemporaneity. Cadmium is a toxic heavy metal frequently found on environment, and his contamination is so frequent as by lead or mercury. Saliva is very-known method to evaluate the cadmium levels on the body. Objective: This study aims to establish technical and structural parameters for the construction of a platinum counter electrode assembly for an electrochemical cell, in order to facilitate their montage, as well to perform the analysis of the sample of saliva in smokers, non-smokers and former smokers, and evaluate the cadmium levels on different cigarettes, cigars, and tobacco brands avaiable on trade. Method: Saliva samples were collected for determination of cadmium by electrochemical analysis. Participants contributed with 1 ml of unstimulated saliva. Tobacco was prepared by immersion of samples on saline for 7 days. To test the platinum electrode, the electrochemical cell was set up with two electrodes (carbon paste as work-electrode and Ag/AgCl as reference-electrode) and the platinum electrode described above, performing the analysis of standard solutions of chloride cadmium in various concentrations by cyclic voltammetry. Voltammogram obtained by the analysis of the solutions was examined using software tools for comparisons with samples of cadmium standard solutions. Results: Tested electrode has performed a satisfactory voltammogram at various concentrations of cadmium, indicating that the electrode worked properly in the electrochemical cell. The saliva tests showed cadmium presence in the patients, showing pH variations and substance concentrations. Tobacco results showed that all the samples had cadmium on his composition. Conclusion: It was observed that homemade electrodes construction is an available assembly electrochemical cell alternative. Similarly, we observed that habit of smoking, along with other occupational factors, increased saliva cadmium levels, aided by cadmium present on tobacco.
Division: IADR/LAR General Session
Meeting:2012 IADR/LAR General Session (Iguaçu Falls, Brazil) Location: Iguaçu Falls, Brazil
Year: 2012 Final Presentation ID:620 Abstract Category|Abstract Category(s):Pharmacology/Therapeutics/Toxicology
Authors
Parisotto, Angelo
( Ponta Grossa State University, Ponta Grossa, N/A, Brazil
)
Molinari Machado, Germano
( Ponta Grossa State University, Ponta Grossa, N/A, Brazil
)
Moro, Evandro
( Ponta Grossa State University, Ponta Grossa, N/A, Brazil
)
Schreiner, Fabiane
( Ponta Grossa State University, Ponta Grossa, N/A, Brazil
)
Kozlowski, Vitoldo Antonio
( Ponta Grossa State University, Ponta Grossa, N/A, Brazil
)
SESSION INFORMATION
Poster Session
Pharmacology/Therapeutics/Toxicology II
06/21/2012