Effects of Fluoride Compounds on Normal Oral and OSCC Cells
Objective: Comparative study of the growth inhibition by different types of fluoride compounds used in the dentistry has been limited. We investigated the effects of three fluoride compounds, sodium fluoride (NaF), diammine silver fluoride [Ag(NH3)2F] and 5-fluorouracil (5-FU), on the growth of a total of eleven human normal and tumor cells.
Method: Viable cell number was determined by MTT method. Apoptosis induction was evaluated by caspase-3 activation and DNA fragmentation. Fluoride was determined by a fluoride-specific electrode.
Result: All compounds showed little or no growth stimulating effect (hormesis) on all cells. Ag(NH3)2F showed the highest cytotoxicity against both normal and tumor cells. 5-FU showed the selective cytostatic activity against oral squamous cell carcinoma (OSCC) cell lines, whereas NaF showed selectivity against glioblastoma cell lines. All compounds did not induce apoptosis markers in a OSCC cell line (HSC-2). Cytotoxicity of all fluoride compounds was not reduced by superoxide dismutase and catalase, reducing the possibility of the involvement of reactive oxygen species. Approximately 0.01~0.09% initially added NaF was recovered from the cells, whereas the cellular uptake of Ag(NH3)2F and 5-FU was below the detection limit.
Conclusion: Cytotoxicity of fluoride compounds may not be directly linked to their tumor-specificity nor apoptosis-inducing activity.
Division: IADR/LAR General Session
Meeting:2012 IADR/LAR General Session (Iguaçu Falls, Brazil) Location: Iguaçu Falls, Brazil
Year: 2012 Final Presentation ID:616 Abstract Category|Abstract Category(s):Pharmacology/Therapeutics/Toxicology
Authors
Mena Acra, Alejandro
( Faculty of Dentistry, Autonomous University of State of Mexico, Toluca, N/A, Mexico
)
Watanabe, Shigeru
( Meikai University, Sakado, Saitama, N/A, Japan
)
Visoso, Angel
( Faculty of Dentistry, Autonomous University of State of Mexico, Toluca, N/A, Mexico
)
Montiel, Norma M.
( Faculty of Dentistry, Autonomous University of State of Mexico, Toluca, N/A, Mexico
)
Sakagami, Hiroshi
( Meikai University, Sakado, Saitama, N/A, Japan
)
Matsuta, Tomohiko
( Meikai University, Sakado, Saitama, N/A, Japan
)
Adachi, Kazunori
( Meikai University, Sakado, Saitama, N/A, Japan
)
Nakajima, Hiroshi
( Meikai University, Sakado, Saitama, N/A, Japan
)
Ogihara, Takashi
( Meikai University, Sakado, Saitama, N/A, Japan
)
Watanabe, Koji
( Meikai University, Sakado, Saitama, N/A, Japan
)
SESSION INFORMATION
Poster Session
Pharmacology/Therapeutics/Toxicology II
06/21/2012