IADR Abstract Archives

Salivary, GCF and Serum Biomarkers in Normal and Obese Subjects

Objectives: To quantify biomarkers for obesity in saliva, GCF and serum to determine if saliva can be used as a biomarker for changes associated with obesity.
Methods: The study was approved by the Human Research Ethics Committee of King's College London (BDM/14/15-8). Informed written consent was obtained from all participants. Resting whole mouth saliva (UWMS), GCF and serum were collected from twenty normal weight (18.5≥BMI>25) with a mean age of 29 years (range 19-44 years) and twenty obese (BMI ≥30) with a mean age of 30.95 years (range 18-45 years) with equal gender distribution. Magnetic Luminex multiplex array assays and other assays were used to measure the level of all selected biomarkers; adiponectin, leptin, resistin, matrix metalloproteinase-8 (MMP8), matrix metalloproteinase-9 (MMP9), tissue inhibitor of metalloproteinase (TIMP1), myeloperoxidase (MPO), C-reactive protein (CRP), receptor activator of nuclear factor kappa B ligand (RANKL) in samples from each subject.
Results: Matched age normal weight and obese groups had body mass index (BMI) 21.5, 32.2 respectively (p<0.01). Adiponectin, leptin and CRP were highest in serum followed by GCF and the least in UWMS. Resistin, MMP8, MMP9, TIMP1 and MPO showed the highest level in GCF followed by serum and then UWMS. RANKL showed the highest level in GCF followed by serum and then UWMS in obese group, whereas in normal weight group followed by UWMS and the least in serum.
The effect of obesity on the level of biomarkers showed that in obese group, adiponectin was reduced in GCF and serum, whilst leptin was reduced in UWMS. MMP8, TIMP1 and CRP were increased in serum. MPO was increased in all samples. RANKL were reduced in UWMS, whilst in GCF and serum, RANKL was increased. The others showed comparable levels in the obese and normal weight groups. Also same levels of albumin, sIgA and gelatinase were found in UWMS of the two groups.

Conclusions: Whilst obesity affected the GCF flow rate serum markers of obesity were still altered in saliva. In addition, obesity caused a number of periodontal remodelling enzymes to change which may cause teeth to become misaligned.
IADR/PER General Session
2018 IADR/PER General Session (London, England)
London, England
2018
0167
Salivary Research
  • Carpenter, Guy  ( King's College London Dental Institute , London , United Kingdom )
  • Saloom, Hayder  ( Baghdad Dental School , Baghdad , Iraq )
  • Cobourne, Martyn  ( King's College London , London , United Kingdom )
  • None
    Oral Session
    Salivary Biomarkers I
    Wednesday, 07/25/2018 , 09:30AM - 11:00AM