IADR Abstract Archives

The P2X7 Receptor, Cathepsin S and Fractalkine in the Trigeminal Subnucleus Caudalis Signal Persistent Hypernociception in Temporomandibular Rat Joint

Objectives: Temporomandibular joint (TMJ) is frequently involved with rheumatoid arthritis with a high prevalence that could result in a chronic pain state. This study establishes animal experimental models that evaluate the chronicity of albumin-induced arthritis hypernociception in the TMJ.
Methods: Male Wistar rats were sensitized subcutaneously with an injection of Methylated Bovine Serum Albumin (mBSA, 500μg) in an emulsion containing phosphate buffered saline (PBS) and Freund's Complete Adjuvant. mBSA dissolved in Freund's Incomplete Adjuvant was injected in different sites in the back of the rat 7 and 14 days after the first immunization. Twenty-one days after the initial injection, TMJ-arthritis was induced in the animals immunized by intra-articular injection of an mBSA (10mg / TMJ / week) during 3 weeks. After 24 hours, 7 and 14 days after the last mBSA intra-articular injection, the animals were terminally anesthetized and their periaricular tissues and trigeminal subnucleus caudalis was collected to immunohistochemistry and biochemistry analyses.
Results: Intra-articular injection of mBSA (10µg/TMJ/week) during 3 weeks resulted in a persistent inflammatory hypernociception which was characterized by an increase of inflammatory exudate with a significantly elicited protein level of pro-inflammatory cytokines IL-12 and IL-18 in the periarticular tissues. Persistent model of albumin-induced arthritis in the TMJ significantly increased P2X7 protein receptor level, cathepsin S and fractalkine pronociceptive factors in the trigeminal subnucleus caudalis that is confirmed by the immunohistochemistry analyses.
Conclusions: Overall, the results of the present work demonstrated that a persistent model of albumin-induced arthritis in the TMJ leads to the activation of the central nervous system signaling by P2X7/cathepsin S/fractalkine pathway.

IADR/PER General Session
2018 IADR/PER General Session (London, England)
London, England
2018
0152
Neuroscience
  • Clemente-napimoga, Juliana  ( São Leopoldo Mandic Institute and Research Center , Campinas , São Paulo , Brazil )
  • Bonfante, Ricardo  ( State University of Campinas , Campinas , São Paulo , Brazil )
  • Macedo, Cristina  ( São Leopoldo Mandic Institute and Research Center , Campinas , São Paulo , Brazil )
  • Abdalla, Henrique  ( State University of Campinas , Campinas , São Paulo , Brazil )
  • Napimoga, Marcelo  ( Sao Leopoldo Mandic Institute and Research Center , Piracicaba , Brazil )
  • grant #016/16529-9, São Paulo Research Foundation (FAPESP)
    None
    Oral Session
    Orofacial Pain: Assessment, Study and Treatment
    Wednesday, 07/25/2018 , 09:30AM - 11:00AM