Molecular Control of Vascular Formation in Developing Mouse Tooth Germ

Objectives: Interactions between epithelial and mesenchymal tissues are crucial for tooth development. In tooth germ, the detailed mechanism of vascular development remains poorly understood. It is expected that vascular development is controlled by various genetic pathways. To clarify the molecular mechanisms of embryonic vascular development in tooth germ, we analyzed the expression patterns of the signaling molecules during tooth germ develpoment.
Methods:
By using immunohistochemical studies and in situ hybridization, we detect the expression patterns of the signaling molecules during embryonic vascular development in tooth germ.
Results: Signaling molecules were expressed especially in peripheral dental mesenchyme and the dental papilla by immunohistochemical studies and in situ hybridization and also the stage-specific expression patterns of them were observed in developing mouse tooth germ.
Conclusions: In this research, we confirmed the localization of the signaling molecules and the stage-specific gene expression patterns during tooth germ development and these evidences provide the beginning of the elucidation for the detailed mechanism of vascular development of tooth germ.
*This work was supported by JSPS KAKENHI Grant Numbers 22592052, 26462800.