IADR Abstract Archives

Salivary Proteome Modification by Transient Receptor Potential Channel Agonists

Objectives: Flavour compounds that increase salivary secretion or modify the composition of saliva are relevant to the development of treatments for xerostomia, which affects 10 – 30% of the population. The aim of this study was to investigate how the whole mouth saliva (WMS) proteome is affected by TRP (Transient Receptor Potential) channel agonists.
Methods: WMS was collected after mouth-rinsing with water, nonivamide (TRPV1 agonist), cinnamaldehyde (TRPA1 agonist) and propylene glycol (vehicle) at concentrations just above the detection thresholds (determined by the 3-AFC method). The proteomes of collected WMS were analysed by relative-quantitative LC-MS/MS. In a follow up study, nonivamide, cinnamaldehyde, propylene glycol and menthol (TRPM8 agonist) were used at concentrations previously determined to be strong sensory stimuli. WMS was collected before and for two minutes after mouth-rinsing with water and each compound. The proteomes of collected WMS were analysed by TMT-labelled absolute-quantitative LC-MS/MS.
Results: In the first study, 1026 proteins were identified of which 36 showed a significant fold-change from the post-water sample. Clustering analyses (k-means) determined variation was participant dependent with differences seen between participants with each of the mouth-rinses. In the second study, 522 unique proteins were identified. Clustering analyses (k-means) determined that changes in the proteome were more mouth-rinse dependent. Protein fold-changes reflected altered biological process and function of the proteome in a gene ontology analysis. Nonivamide and menthol mouth-rinses increased submandibular gland associated proteins whilst cinnamaldehyde increased parotid gland associated proteins.
Conclusions: We demonstrate that the proteome of WMS varies in its response to different flavour compounds. When the stimuli are at the detection threshold most of the variation is seen between individuals but more strongly stimulating concentrations cause compound specific changes. Furthermore, we demonstrated that these compositional changes reflect biological process and function as well as differences in how the major glands contribute to the stimulated WMS proteome.
IADR/PER General Session
2018 IADR/PER General Session (London, England)
London, England
2018
0166
Salivary Research
  • Houghton, Jack  ( King's College London Dental Institute , London , United Kingdom )
  • Hans, Joachim  ( Symrise AG , Holzminden , Germany )
  • Pesaro, Manuel  ( Symrise AG , Holzminden , Germany )
  • Ley, Jakob  ( Symrise AG , Holzminden , Germany )
  • Proctor, Gordon  ( King's College London Dental Institute , London , United Kingdom )
  • BBSRC BB/L015498/1
    NONE
    Oral Session
    Salivary Biomarkers I
    Wednesday, 07/25/2018 , 09:30AM - 11:00AM